Enzymes and Inhibitors
Agilent Technologies ENDO-O-GLYCOSIDASE
Releases unsubstituted, Ser/Thr-linked GalGalNAc from proteins; used with additional enzymes to remove more complex O-linked structures. Also known asEndo-O-Glycosidase, Endo- N-acetylgalactosaminidase, O-Glycosidase.
New England Biolabs, Inc. T4 RNA Ligase 2, truncated K227Q – 2000 units
T4 RNA Ligase 2, truncated K227Q (T4 Rnl2tr K227Q) specifically ligates the pre-adenylated 5' end of DNA or RNA to the 3' OH end of RNA. The enzyme does not use ATP for ligation but requires pre-adenylated linkers. T4 Rnl2tr K227Q is a point mutant of T4 RNA Ligase 2, truncated (NEB# M0242) . Mutation of K227 in T4 RNA Ligase 2 reduces enzyme lysyl adenylation. This mutation further reduces the formation of undesired ligation products (concatemers and circles) by T4 Rnl2tr, possibly by reducing the trace activity of T4 Rnl2tr in transfer of adenylyl groups from linkers to the 5-phosphates of input RNAs. The exclusion of ATP, use of pre-adenylated linkers, and the reduced enzyme lysyl adenylation activity provide the lowest possible background in ligation reactions. This enzyme has been used for optimized linker ligation for the cloning of microRNAs.
Worthington Biochemical Corporation DIAPHORASE 1 KU
Diaphorase, Code: DILW, Store at 2 deg. to 8 deg.C, Minimum activity: 30U/mg dry weight, Supplied as a dialyzed, lyophilized powder, Unit Definition: 1 Unit equals a decrease in absorbance at 600nm of 1.0 per minute at 25 deg.C, pH 7.5, 1kU
FUJIFILM BIOSCIENCES INC LYSLY ENDOPEPTIDASE 10AU/VIAL
*Orders for this supplier are non-cancellable/non-returnable once they are processed.* Lysyl Endopeptidase cleaves peptide bonds at the carboxy-terminal of both lysine residues and S-aminoethylcysteine residues, making it a valuable tool for analysis of peptide and protein primary structure and for enzymatic synthesis of Lys-X compounds. Also, it can retain complete activity after incubation in 4M urea or in 0.1% SDS solution. Pkg. Size:1 vial (10 AU), Preparation: A 10AU-vial contains Tris-HCl corresponding to 1mL of 2mm Tris HCl buffer (pH 8.0),MW: 27,000 (gel filtration); 30,000 (SDS electrophoresis)
Sigma Aldrich Fine Chemicals Biosciences Ribonuclease A from bovine pancreas for molecular biology, 70Kunitz units/mg protein, lyophilized, 9001-99-4, MFCD00132181
Ribonuclease A from bovine pancreas for molecular biology, 70Kunitz units/mg protein, lyophilized
Abcam Carbonic Anhydrase 1/CA1 Inhibitor Screening Kit (Colorimetric)
Carbonic Anhydrase 1/CA1 Inhibitor Screening Kit (Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
New England Biolabs, Inc. Exonuclease VIII, truncated – 1000 units
Exonuclease VIII, truncated, is able to initiate nucleotide removal from the 5' termini of linear double-stranded DNA in the 5' to 3' direction.
New England Biolabs, Inc. RNase HII – 250 units
Ribonuclease HII (RNase HII) is an endoribonuclease that preferentially nicks 5' to a ribonucleotide within the context of a DNA duplex. The enzyme leaves 5' phosphate and 3' hydroxyl ends. RNase HII will also nick at multiple sites along the RNA portion of an Okazaki fragment.
Vector Biolabs Deoxyribonuclease I Adenovirus (Ad-DNASE I)
Ready to use in vivo grade AAV and Adenovirus Ready to use in vivo grade AAV and Adenovirus pre synthesized DNA vector High titer viral vectors 1/EA
Agilent Technologies GLYKO SIALIDASE ASAMPLER KIT
Try our lower MW isoforms for challenging sialidase digests.This Sampler Kit contains 1 vial each of 3 isoforms (88, 66 and 51 kDa) of the enzyme Sialidase A. Lower MW sialidases may help desialylate glycans if steric hindrance is an issue with regular sialidase.